Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

T Cell Reprogramming against Cancer.- Identification of Cell Surface Targets for CAR T Cell Immunotherapy.- Microfluidic Approach for Highly Efficient Viral Transduction.- Single-cell Cytokine Analysis to Characterize CAR-T Cell Activation.- Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells.- Engineering of Natural Killer Cells for Clinical Application.- Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells.- In vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection.- Immunomodulation of NK Cell Activity.- An Overview of Advances in Cell-based Cancer Immunotherapies Based on the Multiple Immune-cancer Cell Interactions.- Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy.- Reprogramming Exosomes for Immunotherapy.- Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor Microenvironment.- CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow Derived Macrophages.- Genome-Wide CRISPRi/a Screening in an In Vitro Co-culture Assay of Human Immune Cell with Tumor Cells.- The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV).- Radiolabeling and Imaging of Adoptively Transferred Immune Cells by Positron Emission Tomography.- Functional Analysis of Human Hematopoietic Stem Cells in vivo in Humanized Mice.- Monitoring Allogeneic CAR-T Cells using Flow Cytometry.- Key Factors in Clinical Protocols for Adoptive Cell Therapy in Melanoma.- Place of Academic GMP Facilities in Modern Cell Therapy.