Clinical Applications of Capillary Electro...

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Beschreibung

The term electrophoresis was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius. In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separationszone, IEF, and isotachophoresishave their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.

Inhaltsverzeichnis

Clinical Applications of Capillary Electrophoresis.- Serum Protein Electrophoresis.- Urine Proteins.- Electrophoresis of Cerebrospinal Fluid.- Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine.- Analysis and Classification of Serum Cryoglobulins.- Myoglobin Analysis.- Enzyme Analysis.- Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis.- Laboratory Diagnosis of Structural Hemoglobinopathies and Thalassemias by Capillary Isoelectric Focusing.- Serum Apolipoproteins.- Gene Dosage in Capillary Electrophoresis.- Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection.- Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis.- Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophoresis.- Serum Lamotrigine Analysis.- Acetonitrile Stacking.- Confirmation of the Presence of Drugs of Abuse in Urine.- Steroid Analysis by Micellar Electrokinetic Capillary Chromatography.- Determination of Polyamines by Capillary Electrophoresis.- Urinary Oxalate and Citrate.- Plasma Nitrite and Nitrate Determination.

Details

Erscheinungsjahr:	2013
Fachbereich:	Andere Fachgebiete
Genre:	Importe , Medizin
Rubrik:	Wissenschaften
Medium:	Taschenbuch
Reihe:	Methods in Molecular Medicine
Inhalt:	xii 212 S. 32 s/w Illustr. 212 p. 32 illus.
ISBN-13:	9781489941749
ISBN-10:	1489941746
Sprache:	Englisch
Einband:	Kartoniert / Broschiert
Autor:	Palfrey, Stephen M.
Redaktion:	Palfrey, Stephen M.
Herausgeber:	Stephen M Palfrey
Auflage:	Softcover reprint of the original 1st edition 1999
Hersteller:	Humana Methods in Molecular Medicine
Verantwortliche Person für die EU:	Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße:	229 x 152 x 13 mm
Von/Mit:	Stephen M. Palfrey
Erscheinungsdatum:	17.08.2013
Gewicht:	0,337 kg