Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains keynotes and implementation advice from the experts

How Genetics has Helped Piece Together the MAPK Signaling Pathway.- In Vitro Enzyme Kinetics Analysis of EGFR.- High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells.- Structural Studies of ERK2 Protein Complexes.- Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk.- Assaying Activation and Subcellular Localization of ERK in Cells and Tissues.- Detection and Functional Analysis of SUMO-Modified MEK.- Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag.- High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates.- Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE.- Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation.- Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions.- The Nuclear Translocation of ERK.- Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA).- Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors.- Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers.- Co-Culture Activation of MAP Kinase in Drosophila S2 Cells.- Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling.- 3D Organotypic Culture Model to Study Components of ERK Signaling.- Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway.- Genome-Wide Analysis of RAS/ERK Signaling Targets.- Probing Chromatin Modifications in Response to ERK Signaling.- Analyzing pERK Activation during Planarian Regeneration.- Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development.- Reconstructing ERK Signaling in the Drosophila Embryofrom Fixed Images.- Using CRISPR-Cas9 to Study ERK Signaling in Drosophila.- Analyzing ERK Signal Dynamics during Zebrafish Somitogenesis.- Modeling RASopathies with Genetically Modified Mouse Models.- Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.