Handbook of Single-Molecule Biophysics

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Sprache: Englisch

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Beschreibung

During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at the fundamental level of sensitivity, that of a single molecule, has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them.

Editorial Advisory Board: Daniel Müller, Cheng Zhu, Claus Seidel, Xiaowei Zhuang, Thomas Schmidt, Nynke Dekker.

Zusammenfassung

Describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping

Addresses the use of single-molecule techniques in super-resolution and functional imaging

Includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells

Integrates single-molecule biophysics and nanoscience

Inhaltsverzeichnis

Single-Molecule Fluorescent Particle Tracking.- Single-Molecule Analysis of Biomembranes.- Single-Molecule Imaging in Live Cells.- Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy.- Single-Molecule FRET: Methods and Biological Applications.- Single-Molecule Enzymology.- Single-Molecule Studies of Rotary Molecular Motors.- Fluorescence Correlation Spectroscopy in Living Cells.- Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy.- Single-Molecule Studies of Nucleic Acid Interactions Using Nanopores.- Nanopores: Generation, Engineering, and Single-Molecule Applications.- Single-Molecule Manipulation Using Optical Traps.- Magnetic Tweezers for Single-Molecule Experiments.- Folding of Proteins under Mechanical Force.- Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy.- Probing Single Membrane Proteins by Atomic Force Microscopy.- High-Speed Atomic Force Microscopy.- Recognition Imaging Using Atomic Force Microscopy.- Atomic Force Microscopy of Protein-Protein Interactions.- A New Approach to Analysis of Single-Molecule Force Measurements.- Single-Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation.

Details

Erscheinungsjahr:	2009
Fachbereich:	Biophysik
Genre:	Biologie , Importe
Rubrik:	Naturwissenschaften & Technik
Medium:	Buch
Inhalt:	xxi 626 S.
ISBN-13:	9780387764962
ISBN-10:	0387764968
Sprache:	Englisch
Herstellernummer:	12111121
Einband:	Gebunden
Redaktion:	Oijen, Antoine van Hinterdorfer, Peter
Herausgeber:	Peter Hinterdorfer/Antoine van Oijen
Hersteller:	Springer US Springer New York
Verantwortliche Person für die EU:	Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße:	241 x 160 x 38 mm
Von/Mit:	Antoine van Oijen (u. a.)
Erscheinungsdatum:	24.07.2009
Gewicht:	1,251 kg