2. Practice of Bright-field Microscopy

3. Preparation of Smears and Simple Stain Practice

4. Gram Stain Practice

5. Acid-Fast Stain Practice

6. Endospore Stain Practice

7. Aseptic Technique-Bacteria Transfer and Streak Plating

8. Lab Practice Test 1-Identify unknown bacteria using Gram stain

9. Introduction of bacteria medium, Nutritional Requirements (Synthetic and Complex Media) Selective & Differential Media (McConkey, Manitol Salt, Blood agar)

10. Lab Practice Test 2-Dilution technology and quiz

11. Numeration bacteria population by pour-plating

12. Numeration bacteria population by spread-plating

13. External Conditions for bacterial growth (Temperature, pH, oxygen, osmatic)

14. Cultivation of anaerobic bacteria

15. Biochemistry test of bacteria-1 (Urease Test, Carbohydrate Fermentation, catalase test, oxidase test)

16. Biochemistry test of bacteria-2 (Nitrate Reduction, Decarboxylation & Deamination of Amino Acids, IMViC Test, Hydrogen Sulfide, Motility and Indole Test)

17. Biochemistry test of bacteria-3 (API20e and enterotube II)

18. Extracellular Enzymatic Activities of Microorganisms

19. Case study 1 (Bacteria cause upper respiratory tract diseases)

20. Clinical lab practice 2-Urine sample bacteria isolation and numeration

21. Case study 2 (Bacteria cause intestinal tract and urine tract diseases)

22. Isolation of phage and PFU determination

23. Antibiotic Sensitivity Testing and Evaluation of Antiseptics/Disinfectants

24. Microbial Counts of Food Product

25. Total Plate Count & Coliform Count of Pond Water