Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Fluorescent Labeling of the Nuclear Envelope without Relying on Inner Nuclear Membrane Proteins.- Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools.- Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging.- A Murine Bone Metastasis Model Using Caudal Artery Injection and Bioluminescence Imaging.- A New Lineage of Artificial Luciferases for Mammalian Cell Imaging.- Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems.- Quantitative Determination and Imaging of Gq Signaling in Live Cells via Split-Luciferase Complementation.- A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules.- Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semi-Rational Molecular Design and Molecular Evolution.- Near Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.- Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells.- Luciferase-Specific Coelenterazine Analogues for Optical Crosstalk-Free Bioassays.- Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe.- Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe.- Live-Cell Fluorescence Imaging of Microtubules by Using a Tau-Derived Peptide.- Discovery of the Environment-Sensitive Near-Infrared (NIR) Fluorogenic Ligand for 1-Adrenergic Receptors Imaging In Vivo.- Rapid and Sensitive Detection of Cancer Cells with Activatable Fluorescent Probes for Enzyme Activity.- Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein.- Development of Near-Infrared Fluorescent Mg2+ Probe and Application to Multi-Color Imaging of Intracellular Signals.- Long-Term Mg2+ Imaging in Live Cells with a Targetable Fluorescent Probe.- Highly Bright NIR-BRET System for Imaging Molecular Events in Live Cells.- Ligand-Activatable BRET9 Probes for Imaging Molecular Events in Living Mammalian Cells.- Manipulation of Actin Cytoskeleton by Intracellular-Targeted ROS Generation.- Bioluminescence Imaging of Neuronal Network Dynamics Using Aequorin-Based Calcium Sensors.- Method for Detecting Emission Spectral Change of Bioluminescent Ratiometric Indicators via a Smartphone.- Bioluminescence Resonance Energy Transfer (BRET) Imaging in Living Cells: Image Acquisition and Quantification.- GPCR Signaling Regulation in Dictyostelium Chemotaxis.- Live Cell Imaging Technique to Visualize DAMPs Release during Regulated Cell Death.- Time-Lapse Imaging of Necroptosis and DAMP Release at Single Cell Resolution.- High-Throughput Whole Plate Imaging of Cells for Multiple Biological Applications.- Pinhole Closure Improves Spatial Resolution in Confocal Scanning Microscopy.- Total Workflows of the Single-Molecule Imaging Analysis in Living Cells: Tutorial Guidance to the Measurement of the Drug Effects on a GPCR.