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Microfluidics Diagnostics
Methods and Protocols
Buch von Valérie Taly (u. a.)
Sprache: Englisch

196,95 €*

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Beschreibung
This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.
This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.
Inhaltsverzeichnis

Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids.- DNA Sizing with pg/mL Sensitivity for Cell-Free DNA Analysis with the mLAS Technology.- On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples.- Isolation of Extracellular Vesicles from Cell Culture and Blood through Nano-Targeted DLD Microfluidic Device.- Microchip for immunomagnetic sorting of Circulating Tumor Cells (CTCs).- Enhancing the Efficiency of Conventional Surface Immunoassays within Standard Labware Using Microscale Flows.- Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis.- Microfluidic Cartridge for Bead-Based Affinity Assays.- A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level.- Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets.- A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption.- An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics.- Microfluidic 3D Cytotoxic Assay.- Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach.- Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types.

Details
Erscheinungsjahr: 2024
Fachbereich: Technik allgemein
Genre: Importe, Technik
Rubrik: Naturwissenschaften & Technik
Medium: Buch
Reihe: Methods in Molecular Biology
Inhalt: xii
255 S.
4 s/w Illustr.
70 farbige Illustr.
255 p. 74 illus.
70 illus. in color. With online files/update.
ISBN-13: 9781071638491
ISBN-10: 1071638491
Sprache: Englisch
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Redaktion: Taly, Valérie
Perez-Toralla, Karla
Descroix, Stéphanie
Herausgeber: Valérie Taly/Stéphanie Descroix/Karla Perez-Toralla
Hersteller: Springer US
Springer New York
Springer US, New York, N.Y.
Methods in Molecular Biology
Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße: 260 x 183 x 21 mm
Von/Mit: Valérie Taly (u. a.)
Erscheinungsdatum: 17.05.2024
Gewicht: 0,707 kg
Artikel-ID: 128516567
Inhaltsverzeichnis

Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids.- DNA Sizing with pg/mL Sensitivity for Cell-Free DNA Analysis with the mLAS Technology.- On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples.- Isolation of Extracellular Vesicles from Cell Culture and Blood through Nano-Targeted DLD Microfluidic Device.- Microchip for immunomagnetic sorting of Circulating Tumor Cells (CTCs).- Enhancing the Efficiency of Conventional Surface Immunoassays within Standard Labware Using Microscale Flows.- Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis.- Microfluidic Cartridge for Bead-Based Affinity Assays.- A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level.- Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets.- A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption.- An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics.- Microfluidic 3D Cytotoxic Assay.- Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach.- Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types.

Details
Erscheinungsjahr: 2024
Fachbereich: Technik allgemein
Genre: Importe, Technik
Rubrik: Naturwissenschaften & Technik
Medium: Buch
Reihe: Methods in Molecular Biology
Inhalt: xii
255 S.
4 s/w Illustr.
70 farbige Illustr.
255 p. 74 illus.
70 illus. in color. With online files/update.
ISBN-13: 9781071638491
ISBN-10: 1071638491
Sprache: Englisch
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Redaktion: Taly, Valérie
Perez-Toralla, Karla
Descroix, Stéphanie
Herausgeber: Valérie Taly/Stéphanie Descroix/Karla Perez-Toralla
Hersteller: Springer US
Springer New York
Springer US, New York, N.Y.
Methods in Molecular Biology
Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße: 260 x 183 x 21 mm
Von/Mit: Valérie Taly (u. a.)
Erscheinungsdatum: 17.05.2024
Gewicht: 0,707 kg
Artikel-ID: 128516567
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