Collects stem cell-derived model-based toxicity assays, both classic and cutting-edge

Features contributions from key opinion leaders from academia and industry

Examines the accompanying rationale and example output data necessary for practical and easy-to-use resource

Includes supplementary material: [...]

1. Stem Cell¿Derived Models for Safety and Toxicity Assessments: Present and Future Studies in the ¿Proclinical Space¿

Gary Gintant and Stefan Braam

2. Human Pluripotent Stem Cell Test for Assessing the Potential Teratogen Risk

Sei Kameoka and Eric Chiao

3. Cardiac Action Potential Measurement in Human Embryonic Stem Cell Cardiomyocytes for Cardiac Safety Studies Using Manual Patch-Clamp Electrophysiology

Muthukrishnan Renganathan, Haiyang Wei, and Yong Zhao

4. Automated Patch Clamp Recordings of Human Stem Cell-Derived Cardiomyocytes

Alison Obergrussberger, Claudia Haarmann, Sonja Stölzle-Feix, Nadine Becker, Atsushi Ohtsuki, Andrea Brüggemann, Michael George, and Niels Fertig

5. The CiPA Microelectrode Array Assay with hSC-Derived Cardiomyocytes: Current Protocol, Future Potential

Daniel C. Millard, Mike Clements, and James D. Ross

6. Optogenetic Approach to Cardiotoxicity Screening: Simultaneous Voltage and Calcium Imaging Under Paced Conditions

Graham T. Dempsey and Christopher A. Werley

7. HTS Compatible Voltage- and Ca2+-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems

Ralf Kettenhofen

8. Kinetic Image Cytometry for Predicting Arrhythmias Using Human Stem Cell-Derived Cardiomyocytes

Emily R. Pfeiffer, Ross Whittaker, Raquel Vega, Fabio Cerignoli, Patrick M. McDonough, and Jeffrey H. Price

9. Decoding Ca2+ Signals as a Non-Electrophysiological Method for Assessing Drug Toxicity in Stem-Cell Derived Cardiomyocytes

Christopher H. George and David H. Edwards

10. Combined Impedance and Extracellular Field Potential Recordings from Human Stem Cell-Derived Cardiomyocytes

Alison Obergrussberger, Ulrich Thomas, Sonja Stölzle-Feix, Nadine Becker, Krisztina Juhasz; Leo Doerr, Matthias Beckler, Michael George, and Niels Fertig

11. Edge Detection for Contractility Measurements with Cardiac Spheroids

Christian Zuppinger

12. Contractile Force Readout of hESC-Cardiomyocytes

Carlota Oleaga, Gregg Legters, L. Richard Bridges, Lee Kumanchik, Candace Martin, Yunqing Cai, Mark Schnepper, Christopher W. McAleer, Christopher J. Long, and James J. Hickman

13. In Vitro Cardiotoxicity Investigation Using High Content Analysis and Human Stem Cell-Derived Models

Liz Roquemore, Ariel Kauss, Catherine Hather, Nick Thomas, and Hirdesh Uppal

14. Structural Toxicity: Hypertrophy Models of Human Pluripotent Stem Cell-Derived Cardiomyocytes

Janos Kriston-Vizi, Sian E. Harding, and Gábor Földes

15. Addressing Functional Neurotoxicity Using the Microelectrode Array (MEA)

Udo Kraushaar, Elke Guenther, and Dietmar Hess

16. Micropatterned Co-Cultures of Induced Pluripotent Stem Cell-Derived Hepatocytes and Stromal Cells for Drug Toxicity Studies

Brenton R. Ware and Salman R. Khetani