Provides detailed protocols for a wide variety of SPR applications

Includes comprehensive background information on SPR techniques for better experimental design

Examines a variety of coupling techniques on different sensor surfaces

Highlights background issues involved in SPR assays to avoid common errors frequently found in SPR literature

Presents protocols in clear step by step procedures including the basics of kinetic analysis

Focuses on a wide range of applications for membrane-bound protein interactions, including G protein-coupled receptors

Chapters on the combination of SPR with mass spectrometry (SPR-MS) for quantification and identification of proteins

Written for users of commercially available SPR equipment

Covers the two most common sources of deviation from ideal kinetics: mass transport and surface heterogeneity

Includes supplementary material: [...]

1. Surface Plasmon Resonance: A General Introduction Nico J. de Mol and Marcel J.E. Fischer 2. The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing Peter Schuck and Huaying Zhao 3. Amine Coupling Through EDC/NHS: A Practical Approach Marcel J.E. Fischer 4. High Affinity Immobilization of Proteins Using Biotin- and GST-based Coupling Strategies Stephanie Q. Hutsell, Randall J. Kimple, David P. Siderovski, Francis S. Willard, and Adam J. Kimple 5. A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance Adam J. Kimple, Robin E. Muller, David P. Siderovski, and Francis S. Willard 6. Affinity Constants for Small Molecules from SPR Competition Experiments Nico J. de Mol 7. Surface Plasmon Resonance Signal Enhancement for Immunoassay of Small Molecules John S. Mitchell and Yinqiu Wu 8. High-Throughput Kinase Assay Based on Surface Plasmon Resonance Hiroyuki Takeda, Naoki Goshima, and Nobuo Nomura 9. SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads Beining Chen 10. Carbohydrate-Lectin Interactions Assayed by SPR Eric Duverger, Nathalie Lamerant-Fayel, Natacha Frison, and Michel Monsigny 11. DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films Sara Peeters and Tim Stakenborg 12. Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors Mojca Podlesnik Besenicar and Gregor Anderluh 13. Capture of Intact Liposomes on Biacore Sensor Chips for Protein-Membrane Interaction Studies Vesna Hodnik and Gregor Anderluh 14. Surface Plasmon Resonance Spectroscopy for Studying the Membrane Binding of Antimicrobial Peptides Kristopher Hall and Marie-Isabel Aguilar 15. Surface Plasmon Resonance Spectroscopy in Determination of the Interactions between Amyloid ß Proteins (Aß) and Lipid Membranes Xu Hou, David H. Small, and Marie-Isabel Aguilar 16. Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies Annette Granéli 17. Application of Surface Plasmon Resonance Spectroscopy to Study G-Protein Coupled Receptor Signalling Konstantin E. Komolov and Karl-Wilhelm Koch 18. Integration of SPR Biosensors with Mass Spectrometry (SPR-MS) Dobrin Nedelkov 19. SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry Jonas Borch and Peter Roepstorff