Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Real-time quantitative PCR and fluorescence in situ hybridization for subcellular localization of miRNAs in neurons.- Live-imaging of axonal cargoes in Drosophila brain explants using confocal microscopy.- Whole Endosome Recording of Vesicular Neurotransmitter Transporter Currents.- Quantitative analysis of presynaptic vesicle luminal pH in cultured neurons.- Investigation of synaptic vesicle proteins in rat brain tissue using real-time qPCR.- Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis.- A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting.- Synaptosomes and metamodulation of receptors.- A novel method to monitor GABA loading into synaptic vesicles by combining patch pipette perfusion and intracellular, caged-GABA photolysis in brain slice preparations.- Rapid Isolation of Functional Synaptic Vesicles from Tissues through Cryo-grinding, Ultracentrifugation and Size Exclusion Chromatography.- Isolation of synaptic vesicles from mammalian brain.- Building and Using a Two-Photon Fluorescence Crosscorrelation Spectroscopy Setup including Fluorescence Lifetime Analysis.- Fluorescence Lifetime and Cross-correlation Spectroscopy for Observing Membrane Fusion of Liposome Models Containing Synaptic Proteins.- Synaptic vesicle pool monitoring with synaptopH-Iuorin.- Imaging neuropeptide release at Drosophila neuromuscular junction with a genetically engineered neuropeptide release reporter.- Imaging synaptic glutamate release with two-photon microscopy in organotypic slice cultures.- Dynole 34-2 and Acrylo-Dyn 2-30, novel dynamin GTPase chemical biology probes.- Synthesis of Pthaladyn-29 and Napthalimide-10, GTP site directed dynamin GTPase inhibitors.